NR AQGY
AU Andreoletti,O.; Simon,S.; Lacroux,C.; Morel,N.; Tabouret,G.; Chabert,A.; Lugan,S.; Corbiere,F.; Ferre,P.; Foucras,G.; Laude,H.; Eychenne,F.; Grassi,J.; Schelcher,F.
TI PrPsc accumulation in myocytes from sheep incubating natural scrapie
QU Nature Medicine 2004 Jun; 10(6): 591-3
PT journal article
AB Because variant Creutzfeldt-Jakob disease (vCJD) in humans probably results from consumption of products contaminated with tissue from animals with bovine spongiform encephalopathy, whether infectious prion protein is present in ruminant muscles is a crucial question. Here we show that experimentally and naturally scrapie-affected sheep accumulate the prion protein PrPsc in a myocyte subset. In naturally infected sheep, PrPsc is detectable in muscle several months before clinical disease onset. The relative amounts of PrPsc suggest a 5,000-fold lower infectivity for muscle as compared to brain.
MH Animals; Cattle; Enzyme-Linked Immunosorbent Assay; Human; Immunohistochemistry; Muscle Fibers/cytology/*metabolism; Nerve Fibers/metabolism; PrPsc Proteins/*metabolism/pathogenicity; Scrapie/*metabolism; Sheep; Support, Non-U.S. Gov't
AD O. Andréoletti, C. Lacroux, G. Tabouret, A. Chabert, S. Lugan, F. Corbière, G. Foucras, F. Schelcher, UMR INRA ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France; S. Simon, N. Morel, J. Grassi, CEA, Service de Pharmacologie et d'Immunologie, DRM, CEA Saclay, 91191 Gif sur Yvette Cedex, France; P. Ferré, UMR INRA ENVT 181, Pharmacologie Toxicologie Expérimentale, Ecole Nationale Vétérinaire de Toulouse, 23 Chemin des Capelles, 31076 Toulouse, France; H. Laude, INRA, Virologie Immunologie Moléculaires, INRA Domaine de Vilvert, 78350 Jouy-en-Josas, France; F. Eychenne, INRA Domaine de Langlade, 31450 Pompertuzat, France; Correspondence should be addressed to O Andréoletti o.andreoletti@envt.fr
SP englisch
PO USA
EA pdf-Datei und zusätzliche Angaben
ZF kritische Zusammenfassung von Dr. Ingrid Schütt-Abraham