NR AYHV
AU Murayama,Y.; Yoshioka,M.; Okada,H.; Takata,M.; Yokoyama,T.; Mohri,S.
TI Urinary excretion and blood level of prions in scrapie-infected hamsters
QU Journal of General Virology 2007 Oct; 88(10): 2890-8
PT journal article; research support, non-u.s. gov't
AB Prions, infectious agents causing transmissible spongiform encephalopathy (TSE), are composed primarily of the pathogenic form (PrPsc) of the host-encoded prion protein. Although very low levels of infectivity have been detected in urine from scrapie-infected rodents, no reports of urinary PrPsc have been substantiated. Studies on the dynamics of urinary PrPsc during infection are needed to ensure the safety of urine-derived biopharmaceuticals and to assess the possible horizontal transmission of prion diseases. Using the protein misfolding cyclic amplification technique, a time-course study of urinary excretion and blood levels of PrPsc was performed in Sc237-infected hamsters and a high rate of PrPsc excretion was found during the terminal stage of the disease. Following oral administration, PrPsc was present in all buffy coat samples examined; it was also present in most of the plasma samples obtained from hamsters in the symptomatic stage. PrPsc was excreted in urine for a few days after oral administration; subsequently, urinary PrPsc was not detected until the terminal disease stage. These results represent the first biochemical detection of PrPsc in urine from TSE-infected animals.
IN Mit der besonders sensitiven der zyklischen PrPsc-Amplifizierung untersuchten Murayama et al. zu verschiedenen Zeitpunkten das Blut und den Urin von Hamstern, die oral mit dem Hamster-adaptierten Scrapie-Stamm Sc237 inokuliert worden waren. 2007 berichteten sie von PrPsc-Nachweisen im Urin frisch inokulierter und bereits erkrankter Hamster. In der buffy coat genannten Schicht abgestandenen Blutes fanden sie zu jedem Zeitpunkt PrPsc, in Serum nur bei erkrankten Hamstern.
MH Animals; Blotting, Western; Brain/pathology; Cricetinae; Functional Laterality; Gene Amplification; PrPsc Proteins/*blood/genetics/metabolism/*urine; Prions/*blood/isolation & purification/*urine; Protein Folding; Scrapie/*blood/genetics/pathology/*urine
AD Prion Disease Research Center, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. ymura@affrc.go.jp
SP englisch
PO England