NR AYHP
AU Lobao-Soares,B.; Walz,R.; Carlotti,C.G.Jr.; Sakamoto,A.C.; Calvo,F.; Terzian,A.L.; da Silva,J.A.; Wichert-Ana,L.; Coimbra,N.C.; Bianchin,M.M.
TI Cellular prion protein regulates the motor behaviour performance and anxiety-induced responses in genetically modified mice
QU Behavioural Brain Research 2007 Oct 1; 183(1): 87-94
PT comparative study; journal article; research support, non-u.s. gov't
AB The cellular prion protein (PrPc) is a sialoglycoprotein involved in neuroplasticity processes and synaptic transmission. This study investigated behavioural responses (balance in the rota-rod test at 24 rpm, motility in the open-field test, anxiety in the elevated plus-maze test) in Zürich developed wild-type adult mice (WT, controls of normal PrPc expression), in knockout (KO) mice (Prnp(0/0), with no PrPc expression), and in PrPc overexpressing Tg-20 mice. After 8 min in the rota-rod test, Tg-20 animals presented significantly fewer falls (1.08+/-1.56 falls) than both WT (7.27+/-4.36) and KO (7.6+/-6.15) mice (p<0.01). In the open field test, Tg-20 animals showed significantly increased motility [rearing=23.4+/-7.85, crossing=97.30+/-32.11) when compared with KO mice (rearing=5.45+/-3.69 and crossing=59.73+/-15.43) or WT mice (rearing=6.5+/-20.23 and crossing=45.18+/-20.33) (p<0.01). In the elevated plus-maze test, Tg-20 mice showed less anxiety (head projections=7.3+/-1.62) when compared with WT animals (3.38+/-0.67) (p<0.05). Moreover, KO mice spent more time in the centre of the plus maze (37.80+/-5.57 s) than did WT mice (22.57+/-3.82) (p<0.05). PrPc overexpressing mice evoked increased motility, less anxiety, and increased equilibrium when compared with WT control animals in the behavioural protocols used. KO animals also tended to evoke fewer anxiety-related responses in the elevated plus-maze test. These findings indicate that the levels of PrPc in adult life are associated with possible changes in motility, anxiety, and equilibrium.
MH Adaptation, Psychological/physiology; Analysis of Variance; Animals; Anxiety/*metabolism; Exploratory Behavior/*physiology; Male; Mice; Mice, Inbred Strains; Mice, Knockout; Mice, Transgenic; Motor Activity/*physiology; Motor Skills/physiology; PrPc Proteins/genetics/*metabolism; Rotarod Performance Test; Species Specificity; Statistics, Nonparametric
AD Laboratorio de Neuroanatomia & Neuropsicobiologia, Departamento de Farmacologia, Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo (USP), Av. dos Bandeirantes, 3900, Ribeirao Preto, SP 14049-900, Brasil.
SP englisch
PO Niederlande