NR AYCK
AU Martinez,T.; Pascual,A.
TI Identification of genes differentially expressed in SH-SY5Y neuroblastoma cells exposed to the prion peptide 106-126
QU European Journal of Neuroscience 2007 Jul; 26(1): 51-9
PT journal article; research support, non-u.s. gov't
AB Prion diseases are a group of neurodegenerative disorders characterized by astrocytosis and progressive neuronal degeneration. As a causative agent, prions have been intensely investigated in different experimental models. However, the mechanisms and pathways involved in the prion-induced neurological dysfunction are poorly understood. In this work we have investigated the influence of prion infection on the gene expression profile in a human neuroblastoma cell line. Using a DNA microarray and quantitative reverse transcriptase-polymerase chain reaction methods, we have analysed in SH-SY5Y cells the effects of a synthetic peptide corresponding to the 106-126 neurotoxic region of the cellular human prion protein. Our results show that addition of this peptide to the neuronal culture specifically changes the expression of a relative high number of genes, and causes a progressive neuronal death even in the absence of microglia.
MH Cell Death/physiology; Cell Line, Tumor; Down-Regulation/genetics/physiology; Gene Expression/*physiology; Humans; Microscopy, Fluorescence; Neuroblastoma/*genetics/*metabolism; Neuroglia/drug effects/metabolism; Oligonucleotide Array Sequence Analysis; Peptide Fragments/*pharmacology; Prions/genetics/*pharmacology; RNA/biosynthesis/genetics; Reverse Transcriptase Polymerase Chain Reaction; Tetrazolium Salts; Thiazoles; Transcription, Genetic
AD Instituto de Investigaciones Biomedicas, Consejo Superior de Investigaciones Cientificas, Madrid, Spain.
SP englisch
PO Frankreich