NR AXYW
AU Willemarck,N.; van Poucke,M.; van Steen,K.; Sofie,M.; van Zeveren,A.; Peelman,L.
TI PRNP Polymorphisms Associated with PRNP mRNA Expression in Blood in Belgian Sheep Population
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.28
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Out of 169 bi-allelic polymorphisms, found by sequencing the complete PRNP gene of 40 sheep showing differential PRNP mRNA expression in blood, 5 single PRNP polymorphisms (4 in intron 2 and 1 in 3'-UTR) were found to be associated with higher PRNP mRNA expression in blood, using a non-parametric multifactor dimensionality reduction (MDR) method.
In order to estimate the frequency of these polymorphisms in the Belgian sheep population, 350 unrelated sheep, constituting 7 different breeds (Ardense Voskop, Bleu du Maine, Hampshire Down, Rouge de l'Ouest, Suffolk, Texel and Vlaams Kuddeschaap), were genotyped.
For each of the 5 polymorphisms an assay (PCR-GE, PCR-RFLP or sequencing) was designed and performed on genomic DNA of 350 sheep, isolated from blood by alkaline lysis.
All 5 polymorphisms could be detected in all tested breeds, except in Bleu du Maine (N=61) and Rouge de l'Ouest (N=28), with an averaged prevalence of 18.02%. Sheep homozygous for the polymorphisms were only found in Vlaams Kuddeschaap (highest prevalence of 30%; N=53). Haplotype analysis shows that all 5 polymorphisms are in strong linkage disequilibrium. Genotyping was also performed on 9 scrapie cases in Belgium (8 atypical and 1 classical; see poster Dobly et al.). Two of the polymorphisms will be used for genotyping a larger number of TSE infected/non-infected sheep in order to detect a possible association with TSE susceptibility/resistance based on the hypothesis that lower mRNA expression results in higher TSE resistance. All polymorphisms are currently subjected to a characterisation assay in vitro to study their role in PRNP mRNA expression.
AD N. Willemarck, M. van Poucke, M. Sofie, A. van Zeveren, L. Peelman, Ghent University, Belgium; K. van Steen, Ghent University Hospital, Belgium
SP englisch
PO Schottland