NR AXYB
AU Ulvund,M.J.; Baardsen,K.; Brundtland,E.; Meling,S.; Ersdal,C.; Espenes,A.; Press,C.McL.
TI A New Experimental Model for Studying Scrapie and Prion Disease in Sheep
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.68
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
A new experimental sheep model for studying the pathogenesis and development of scrapie and prion disease has been developed at the Norwegian School of Veterinary Science (NVH), Norway. At NVH, an experimental sheep model involving oral dosing of 6-8 weeks old lambs has been used to investigate the pathogenesis of scrapie (Ulvund et al. 2005, Ersdal et al. 2005). The need to follow animals for several years has made these studies laborious and time-consuming. This new experimental model involves oral dosing of lambs with infective brain tissue at birth, before the lambs receive colostrum from their mothers. In this pilot study, 13 lambs were inoculated orally with either scrapie infected brain or normal brain material of the same genotype. The lambs were then kept with their mothers in closed isolation facilities, observed by videosurveillance, and euthanized when obvious and typical clinical symptoms of scrapie were observed. All lambs were of the homozygous V136R154Q171 PrP genotype. Vague clinical symptoms appeared gradually from 3-4 months, and at euthanasia, 5 months after dosing, all lambs dosed with infectivity had typical symptoms. Various organs and tissues (six brain areas, the whole spinal cord, various ganglia, lymphoid tissues, adrenals and intestines) were examined by immunohistochemistry with relevant monoclonal or polyclonal anti PrP antibodies (Ersdal et al. 2003, 2005), western blot and gene expression. The results so far show that the model is extremely effective, quick and is well suited for studying pathogenesis. This new experimental approach represents an improved model for studying preclinical diagnosis of prion disease in live sheep (examination of blood, CSF, and biopsy-obtainable tissues).
Ersdal C, Ulvund MJ, Benestad SL, Tranulis MA (2003). Accumulation of pathogenic prion protein (PrPsc) in nervous and lymphoid tissues of sheep with subclinical scrapie. Vet Pathol 40:164-174.
Ulvund MJ, Ersdal C, Espenes A, Press CMcL. An experimental scrapie model in sheep - possibilities and limitations (2005). International Sheep Veterinary Congress, Hersonissos, Greece 313-314.
Ersdal C, Ulvund MJ, Espenes A, Benestad S, Sarradin P (2005) Mapping PrPsc propagation in experimental and natural scrapie in sheep with different PrP genotypes. Vet Pathol 42:258-274.
AD M.J. Ulvund, K. Baardsen, E. Brundtland, S. Meling, C. Ersdal, Norwegian School of Veterinary Science, Small Ruminant Research, Norway; A. Espenes, C.M.C.L. Press, Norwegian School of Veterinary Science, Basic and Aquatic Sciences, Norway
SP englisch
PO Schottland