NR AXXX
AU Tortosa,R.; Costa,C.; Vidal,E.; Castells,X.; Barcelo,A.; Torres,J.M.; Pumarola,M.; Arino,J.
TI Microarray Gene Expression of a Murine Transgenic Model of the Bovine Spongiform Encephalopathy
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.73
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Microarray gene expression is a scientific laboratory tool that allows identifying genes with altered expression patterns in determined time points and circumstances and, consequently, gives information about the possible variation in the levels of the related proteins.
Many human and animal transmissible spongiform encephalopathies have been object of this gene expression analysis, being Scrapie the most studied animal TSE. Bovine spongiform encephalopathy is a neurodegenerative disease that produces fatal effects to the affected animal population. Even though the brain histopathological changes associated to the disease have been well described as well as many processes involved in its pathogenesis, there still are many blanks surrounding this disease. Despite the social and economical importance of BSE, as a zoonotic disease, no microarray gene expression studies have been yet published.
Since these techniques have never been applied to BSE, it was our objective to use them in a transgenic mice model in order to obtain new data of the disease as an orientation for further investigations on the pathogenesis of BSE.
Twenty transgenic mice were inoculated intracerebrally with an infective BSE homogenate and humanely sacrificed at different time points of the disease. Twenty age and time matched controls inoculated with healthy brain homogenate were also sacrificed. The brain of these mice was immediately collected and processed for RNA extraction. DNAs synthesized from the extracted mRNAs were hybridized with Gene Chip microarrays. The resulting data was filtered and statistically analyzed and further comparisons between the control and the infected group revealed changes throughout all the time course of the disease.Some of these results were tested by either RT-PCR or immunohistochemistry techniques using paraffin embedded brain samples.
The observed gene expression changes were indicative of an inflammatory reaction of the brain in advanced stages of the disease. The results also showed changes in neuronal metabolism and functionality from early stages of the encephalopathy.
This study was financed by the project EET2002-05168-C04 of the Spanish Ministry of Science and Technology (MCyT).
AD R. Tortosa, C. Costa, Veterinary Faculty, UAB, Spain; E. Vidal, M. Pumarola, CReSA, Priocat Laboratory, Spain; X. Castells, A. Barceló, J. Ariño, Veterinary Faculty, UAB, Spain; J.M. Torres, INIA, CISA, Spain
SP englisch
PO Schottland
EA pdf-Datei und Poster (Poster: Autorenreihenfolge geändert)