NR AXVN
AU Saba,R.; Siemens,C.; Shannon,H.; Booth,S.
TI MicroRNA Expression Profiling in Prion Disease
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.98
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Global analysis of gene expression in the brains of prion infected humans and animals have led to the identification of numerous genes which exhibit transcriptional alterations associated with disease progression. These genes reflect the pathways and biological processes that characterize the key events in prion pathogenesis. However, a central question is how these genes are controlled. Recent work has revealed that a class of small non-coding RNA species, known as microRNAs (miRNAs) has critical functions across various biological processes, for example miRNAs have been shown to modulate programmed cell death during development.
To address whether miRNAs play a role in prion-induced neurodegeneration, we developed a microarray for miRNA expression profiling and performed an analysis of over 400 mammalian miRNAs from mouse brains infected with scrapie. We identified miRNA molecules that show significant, disease-specific, changes in their levels of expression. These miRNA molecules, in turn, act to control other genes that change in the diseased brain. Interestingly, some of these genes are involved in safeguarding brain cells from stress and maintaining their viability and function. We have introduced these miRNAs into brain cells grown in the laboratory and we will present results outlining the potential regulatory function of these molecules. We have previously reported comprehensive analyses of gene expression, and the biological processes which are affected, in brains of scrapie infected mice. MiRNAs can act as key regulators of these changes and their inclusion into regulatory pathways and networks will provide a more complete picture of the events leading to neurodegeneration.
AD R. Saba, C. Siemens, H. Shannon, S. Booth, National Microbiology Laboratory, Prion Diseases Program, Canada
SP englisch
PO Schottland
EA pdf-Datei und Poster (Postertitel: Prion Induced Deregulation of MicroRNAs (miRNAs) in the Mouse Brain