NR AXVH
AU Robertson,C.; Graham,C.; Balachandran,A.; Parchaliuk,D.; Booth,S.
TI Plasma Derived Exosomes as a Source of Disease Specific Biomarkers
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.100
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Exosomes are small (50-90nm) membrane-bound vesicles released by many cell types including B cells, T cells, dendritic cells, reticulocytes, intestinal epithelial cells, tumour cells, and platelets. Exosomes harbour unique subsets of proteins that are linked to the type, and physiological state, of the cells in which they originate. They have been shown to be capable of transmitting proteins such as MHC-peptide complexes and antigens, from cell to cell. This evidence suggests that exosomes play important roles in intercellular communication and immune regulation and response.
Recent studies in our laboratory, and others, have shown that cellular prion protein (PrPc) is present on exosomes; indeed, exosomes have been shown to be able to transmit infectious prions. These findings have prompted further investigations into the possible role of exosomes in the replication and transport of prions in the body.
In the present study we have purified exosomes from both human and elk plasma and found that they contain relatively high concentrations of PrPc, as well as a number of common markers such as CD9 and CD63. We have compared the protein content of purified exosomes from the plasma of CWD infected elk, with that of exosomes isolated from uninfected animals, using comparative proteomic technologies. The results of these experiments will be described. The protein content of exosomes is characteristic of the disease status of the cells from which they were derived and we believe that they are a readily accessible, enriched source of biomarkers. In addition exosome membranes are very similar to lipid rafts and provide a convenient microenvironment for investigations into prion propagation.
AD C. Robertson, D. Parchaliuk, S. Booth, National Microbiology Laboratory, Prion Diseases Program, Canada; C. Graham, Canadian Food Inspection Agency, TSE Laboratory, Canada; A. Balachandran, Canadian Food Inspection Agency, National Reference Laboratory for Scrapie and Chro, Canada
SP englisch
PO Schottland