NR AXUN
AU Polak,M.P.; Jacobs,J.G.; Zmudzinski,J.F.; Langeveld,J.P.M.
TI Update on the Status of Unusual Isolates of Bovine Spongiform Encephalopathy in Poland and The Netherlands
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Natural and Experimental Strains P02.03
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
B. Recent classification of unusual isolates has improved awareness about additional types of bovine spongiform encephalopathy (BSE). This has led to distinction of H-type and L-type isolates that can be discriminated from common BSE (C-type). The classification of these isolates by reference laboratories will require preferably simple and robust tools. A./O. Using Polish and Dutch archives of BSE isolates and previous experience about typing unusual BSE cases based on properties of proteinase K resistant PrP (PrPres) we looked for convenient parameters to identify those atypical forms of BSE.
M. Confirmed BSE cases - 48 from Poland and 83 from The Netherlands were analyzed by Western blotting with several monoclonal antibodies directed at N terminal and core epitopes of prion protein (PrP). R. Most cases showed the classical glycoprofile characterized by dominance of di- (D) over monoglycosylated (M) PrPres band yielding D/M ratios well above 2 with antibodies having their epitopes in bovine PrP region 109-164 (C-type cases). Eight cases of BSE were classified as L type based on a conspicuously low D/M ratio of glycosylated PrPres bands approaching unity. These L-type cases (like previously described BASE-cases) exhibited also a slightly lower molecular mass (Mr) of the non glycosylated band with respect to C-types. Two cases were classified as H type because of a markedly higher Mr of PrPres when compared with C type cases, and carried an epitope in the 101-105 region of PrP. This type of BSE was only well recognized by antibody 12B2. D. Western blotting of homogenates from BSE infected brain stems after proteinase K digestion using both PrP-epitopes of 12B2 and core antibody together with D/M ratio yields conclusive and straightforward information about the type of BSE (C, L, or H type). The occurrence of L type and H type cases in cows at ages of exclusively 8 years and older raises questions on the prevalence of sporadic forms of prion diseases in cattle and the origin of BSE.
AD
M.P. Polak, J.F. Zmudzinski, National Veterinary Research Institute, Department of Virology, Poland;
J.G. Jacobs, J.P.M. Langeveld, Central Institute for Animal Disease Control (CIDC-Lelystad), Department Bacteriology & TSEs, Netherlands
SP englisch
PO Schottland