NR AXTG
AU Muramatsu,Y.; Hayakawa,H.; Ishokawa,Y.; Takahashi,K.; Horiuchi,M.; Tamura,Y.
TI Surveillance for Frequencies of Polymorphisms on Bovine Prion Protein Gene of Commercial Sires in Japan
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.86
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
The recent discovery of significant associations between bovine spongiform encephalopathy (BSE) susceptibility in cattle and the frequency distributions of insertion/deletion (indel) polymorphisms within the bovine prion protein (PRNP) gene prompted an evaluation of 283 commercial sires in Hokkaido, where dairy farming has prospered the most in Japan. Two variable sites, a 23-bp indel polymorphism (123 bp/100 bp) in promoter region and a 12-bp indel polymorphism (103 bp/91 bp) in intron 1, were investigated by PCR. As for the 23-bp indel in the commercial sires, the frequency distributions of 100 bp allele and 100 bp/100 bp genotype which were
mentioned the association with BSE susceptibility in the BSE-affected German cattle were 54.8% and 29.7%. On the other hand, the frequency distribution of 91 bp allele and 91bp/91bp genotype in 12-bp intron 1 site which were mentioned the association with BSE susceptibility in the UK cattle were 49.6% and 23.0% in the commercial sires. The frequency distributions of 100 bp allele and 100 bp/100 bp genotype in 23bp indel site in Japanese sires were different from those of the United States (U.S.) sires. No significant allelic and genotypic differences were detected for the 12-bp indel on intron 1 region between Japanese sires and the U.S. sires. Our results suggest that Japanese sires have low suspectivility against BSE.
AD Y. Muramatsu, Y. Tamura, Rakuno Gakuen University, Veterinary Public Health, Japan; H. Hayakawa, Y. Ishokawa, K. Takahashi, Genetics Hokkaido, Production, Japan; M. Horiuchi, Hokkaido University, Laboratory of Prion Diseases, Japan
SP englisch
PO Schottland