NR AXRZ
AU Mallinson,G.; Kaisar,M.; Appleford,N.
TI Screening for vCJD in UK NSHSBT Tissue Donors using a Highly Sensitive Western Blot Protocol
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.82
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
A characteristic feature of the pathogenesis of vCJD is an accumulation of the disease associated isoform of the prion protein in the lymphoreticular tissues and brain of affected individuals. Since accumulation of prions in lymphoreticular tissue is likely to occur relatively early, before neurological symptoms become apparent, it is possible that tissue donation may be a route of vCJD transmission. A pilot study to screen UK NHS Blood & Transplant deceased tissue donors for vCJD has been underway since April 2006 using tonsil tissue retrieved at the time of tissue donation. The study will be extended to include spleen tissue in the near future.
The test protocol consists of a prion concentration step developed from the Prionics(R)Check Western kit followed by Western blot analysis. The prion concentration procedure is rapid, robust and highly efficient at recovering prions from vCJD brain homogenate spiked into tonsil and optic nerve tissue homogenate. The protocol is at least as sensitive as sodium phosphotungstate precipitation against brain and spleen tissue homogenates from vCJD affected individuals and could be readily automated if a high throughput screen is required.
AD G. Mallinson, M. Kaisar, N. Appleford, Bristol Institute for Transfusion Sciences, UK
SP englisch
PO Schottland