NR AXQJ
AU Krasemann,S.; Bodemer,W.; Kaup,F.J.; Glatzel,M.
TI Investigation of Preclinical Deposition of PrPsc in Peripheral Tissues of Primates Challenged with vCJD, BSE and sCJD Prions
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.102
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Non-human primates have been shown to be a reliable model for the experimental transmission of human prion diseases showing a comparable clinical course and a typical pathology within the brain tissue of terminally ill animals. However, to define the risk of accidental transmission of prion diseases via non-neuronal tissues the preclinical deposition of PrPsc has to be elucidated. The involvement of extraneural tissues such as lymphoreticular system and muscle had been demonstrated in humans and experimentally infected animals with clinical disease. To investigate whether extraneural tissues play a role in the preclinical phase of human prion diseases we infected rhesus monkeys with sCJD, BSE and vCJD via the intraperitoneal route. Animals were taken at defined time points before and after development of first clinical signs. Central nervous tissue showed deposition of PrPsc in a disease specific pattern. The amount of PrPsc corresponded directly to the duration of infection. We investigated the deposition of PrPsc in different muscle samples (skeletal muscle, heart, tongue, tongue root) using highly sensitive detection methods. Interestingly, the amount of PrPsc was highest within tongue tissue of vCJD infected monkeys and could be detected several month before the development of clinical symptoms.
AD S. Krasemann, M. Glatzel, University of Hamburg-Eppendorf, Inst. of Neuropathology, Germany; W. Bodemer, F.J. Kaup, German Primate Center, Germany
SP englisch
PO Schottland
EA pdf-Datei und Poster (Postertitel: Investigation of preclinical deposition of PrPsc in muscle tissues of primates challenged with vCJD, BSE and sCJD prions)