NR AXQB
AU Knox,J.D.; Simon,S.L.R.; LeMaistre,J.; Lise,L.
TI Prion-Induced Changes in the Urinary Protein Profile
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.127
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB The use of PrPd as a pre-clinical, or general marker for surveillance is limited, due to the fact that it is present in extremely small amounts in accessible tissues, or in body fluids such as cerebrospinal fluid, blood and urine. Specific detection of these small amounts of the PrPd conformer is further exacerbated by the presence of a large excess of endogenous PrP. Thus the identification of alternative biomarkers applicable to the development of diagnostic tests or intervention therapies is needed. Urine, due to its ease of collection and comparatively less complex protein profile, is perhaps the ideal fluid for surveillance provided a sufficiently sensitive and specific alternative biomarker for prion infection can be identified. In this study weekly collections of urine were performed on ME-7 infected C57/BL6 mice and age matched controls. The extracted proteins were analyzed by 2-D fluorescence difference gel electrophoresis (2D-DIGE). Differential in gel analysis using DeCyder software (GE Healthcare) was used to identify differentially expressed proteins throughout disease progression. Gel spots of interest were isolated and identified by tandem mass spectrometry. The pattern of abundance and identity of some of the differentially abundant proteins are indicative of an acute phase response (APR) occurring early in the disease course in infected animals.
AD J.D. Knox, S. Simon, L. Lise, PHAC, Prion Disease Program, Canada; J. LeMaistre, University of Manitoba, Department of Pharmacology, Canada
SP englisch
PO Schottland
EA pdf-Datei und Poster (Posterautoren: L. Lamoureux, M. Plews, M. Stobart, S.L.R. Simon und J.D. Knox)