NR AXPG
AU Johnson,C.; Vanderloo,J.P.; Herbst,A.; Bochsler,P.; Keane,D.; Aiken,J.M.; McKenzie,D.
TI Prion Alleles Influence Disease Progression in Orally Challenged White Tail Deer
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.89
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB We have shown a statistically significant bias in chronic wasting disease (CWD) prevalence in free-ranging Wisconsin white-tailed deer associated with the presence of specific Prion Protein (PrP) alleles. To determine the extent of influence these PrP polymorphisms exert on susceptibility to and progression of CWD, we initiated oral transmission of CWD agent into white-tailed deer. Deer selected for this study had PrP alleles that were variable at amino acids (AA) 95 and 96 with the most common allele having glutamine at position 95 and glycine at position 96, which we refer to as wildtype (wt). Two other alleles were present in the deer used, a glutamine to histidine change at position 95 (Q95H) and a glycine to serine at position 96 (G96S). All twelve deer used in this study were rescue fawns from outside known CWD-positive regions of Wisconsin, and tested negative for CWD by tonsil biopsy. Two deer were lost to nondisease related death. Of the remaining inoculated deer, all wt/wt deer became endstage positive for CWD with an average incubation period of 693 days post inoculation (PI), all 96/wt deer became end-stage positive with an average incubation period of 956 days PI and neither the 95/wt or 95/96 deer have demonstrated any clinical symptoms of CWD as of 5/16/2007, 1101 days PI.
AD C. Johnson, J.P. Vanderloo, A. Herbst, J. Aiken, D. McKenzie, University of Wisconsin, Madison, Comparative Biosciences, USA; P. Bochsler, D. Keane, Wisconsin Veterinary Diagnostic Laboratory, USA
SP englisch
PO Schottland