NR AXNJ
AU Graham,J.F.; Kirby,L.; Gill,A.C.
TI In Vitro Analysis of the Molecular Basis of Strain Variation
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Protein Misfolding P01.19
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB The prion hypothesis states that the infectious agent is predominantly composed of misfolded PrP. One of the challenges to this hypothesis is the existence of prion strains. Different strains of the infectious agent are thought to arise from different conformations of PrPsc that can be faithfully passaged onto PrPc during conversion. To account for the molecular basis behind strains, it has been suggested that there is the participation of strain specific co-factors that aid in the refolding process during conversion. This may also be aided by the preferential targeting of different TSE strains to specific brain regions and potentially different cell types. This project aims to investigate the possible involvement of accessory molecules in strain definition. Potential accessory molecules will be sourced from TSE susceptible cell lines and analysed using the cell free conversion assay. Alternatively, scrapie associated fibrils of mouse scrapie will be analysed by mass spectrometry to identify co-factors that are required by individual prion strains for efficient conversion of PrPc to PrPsc. In vitro refolding assays will form the basis for establishing an assay that promotes refolding of rPrP, with the aid of strain specific co-factors, into a ß-sheet rich isoform that mimics PrPsc from different TSE strains, without seeding with exogenous PrPsc. We will present an update on progress towards achieving our aims.
AD J.F. Graham, L. Kirby, NPU, Roslin Institute, TSE Division, Compton, UK; A.C. Gill, Neuropathogenesis Unit, TSE Department, Compton, UK
SP englisch
PO Schottland
EA pdf-Datei und Poster (zusätzliche Autorin T.J.T. Pinneiro, Titel: In Vitro Analysis of the Molecular Basis of Scrapie Strain Variation)