NR AXLJ
AU Edwards,J.; Horton,R.; Bellworthy,S.; Simmons,H.; Terry,L.
TI Characterisation of Leukocyte Surface PrP Epitope Exposure between PBMCs and Leukocytes from Lymph Nodes of Sheep Affected with Natural Scrapie
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.180
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Scrapie pathogenesis in sheep is known to involve the early accumulation of PrPsc within the lymphoreticular system (LRS), notably by follicular dendritic cells and macrophages within lymph nodes. Transfusion experiments have provided evidence that blood also contains infectivity for TSE disease. The components of blood that bear infectivity are less well understood but there is accumulating evidence that some infectivity is cell associated. In order to determine whether infectivity in blood may be associated with transport of cells from the lymph node, we have examined the PrP expression on cells from lymph nodes and peripheral blood mononuclear cells (PBMCs) at terminal stages of scrapie disease. By using a panel of anti-PrP antibodies directed to a range of PrP epitopes, we have used flow cytometry to analyse the PrP epitope characteristics of these cells both from sheep with naturally acquired scrapie and healthy controls. Comparison of immunostaining of PBMCs from scrapie terminal sheep with age and genotype matched controls indicated that the mean fluorescent intensity and the percentage of positively stained cells were both decreased for the majority of antibodies tested, suggesting that the expression of PrP is decreased on PBMCs from scrapie terminal sheep. Our results also indicate that there are significant differences in cell surface expression of PrPc between leukocytes prepared from peripheral blood and from lymph nodes.
AD J. Edwards, R. Horton, S. Bellworthy, Hugh Simmons, L. Terry, Veterinary Laboratories Agency, UK
SP englisch
PO Schottland
EA pdf-Datei und Poster (Postertitel: Cell surface PrP epitope exposure on PBMCs and leucocytes from lymph nodes of sheep naturally infected with scrapie)