NR AXLH
AU Eaton,S.L.; Anderson,M.J.; Rocchi,M.S.; Hamilton,S.; Finlayson,J.; Steele,P.J.; Gonzalez,L.; Dagleish,M.P.; Jeffrey,M.; Reid,H.W.; Chianini,F.
TI Early Immune Events Following Experimental Scrapie Infection in Sheep
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.82
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB In order to gain a greater understanding of the pathogenesis of natural scrapie in sheep an experimental model was developed to investigate immune system cells in the minutes proceeding scrapie infection. Four susceptible (ARQ/ARQ) one year old Romney sheep were inoculated at four different peripheral lymph node drainage sites. The lymph nodes were surgically removed at specific time points; submandibular lymph node (LN) at 300 minutes post infection (m.p.i), prescapular LN at 180 m.p.i., prefemoral LN at 90 m.p.i. and the popliteal LN at 30 m.p.i.. Inoculation was delivered via the subcutaneous route with clarified scrapie brain homogenate injected into the left hand side LNs and clarified scrapie-free brain homogenate into the right hand side. Flow cytometric triple labelling was performed on the LN cells to simultaneously detect lymphocytes, prion protein and the major histocompatability complex II (MHC II) on the cell surface. In addition, immunohistochemistry was used to detect the abnormal form of prion protein (PrPd) in the resected lymph node samples. Preliminary results show PrPd was not detectable in the LN samples at any of the time points studied. Flow cytometry results have suggested that the level of PrP and MHC II co-expression on the cell surface differs depending upon the cell phenotype. CD14+ and CD21+ cells expressed PrP and MHC II simultaneously whereas co-expression was rarely detected on the CD4+ and gamma delta T cells cell surface.
AD S.L. Eaton, M.S. Rocchi, S. Hamilton, J. Finlayson, P.J. Steele, M.P. Dagleish, H.W. Reid, F. Chianini, Moredun Research Institute, UK; M.J. Anderson, University of Edinburgh, UK; L. González, M. Jeffrey, Veterinary Laboratory Agency, Lasswade, UK
SP englisch
PO Schottland
EA pdf-Datei und Poster (Posterautoren: S.L. Eaton, M.J. Anderson, M.S. Rocchi, S. Hamilton, L. Gonzalez, M. Jeffrey, H.W. Reid und F. Chianini)