NR AXLA
AU Dong,X.P.; Chen,J.M.; Gao,C.; Shi,Q.; Shan,B.; Lei,Y.J.; Dong,C.F.; An,R.; Wang,G.R.; Zhang,B.Y.; Han,J.
TI Different Expression Levels of CK2 Subunits in the Brains of Animal Models Infected with Scrapie Agent 263K or 139A and Human TSE
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.109
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Background: Protein kinase, casein kinase 2 (CK2) is a highly conserved serine/threonine protein kinase which is ubiquitous in eukaryotic organisms. In some neurodegenerative diseases, e.g. Alzheimer disease (AD), the amount and activity of CK2 were reported to be decreased.
Objective: To address the possible alteration of CK2 in transmissible spongiform encephalopathies (TSEs).
Methods: The levels of CK2 in the brain tissues of the hamsters inoculated intracerebrally with scrapie agents 263K or 139A, as well as the C57BL mice infected with scrapie agent 139A were evaluated by Western blots, following quantitative analysis of immunoblot images.
Results: Compared with normal animals, the levels of CK2alpha and CK2ß in the brains of infected hamsters and mice were significantly decreased, regardless of scrapie agents. In addition, the expressions of CK2alpha' in the hamsters infected with agents 263K or 139A, and CK2alpha'/CK2alpha'' in the mice infected with agent 139A, were remarkably increased. Comparative analyses on the presences of CK2alpha' or CK2alpha'' in the brains of animals infected with scrapie illustrated an obviously species-related, but not strainrelated manner. Furthermore, decreases of CK2alpha and increases of CK2alpha'/CK2alpha'' were observed in cerebella homogenates from one familial Creutsfeldt-Jakob disease (fCJD) case and one fatal familial insomnia (FFI) case.
Conclusion: These results firstly suggested that alterations of CK2 subunits in brains were illness-correlative phenomenon in TSEs and proposed the potential linkage of CK2 changes with the pathogenesis of prion diseases.
AD X.-P. Dong, State Key Lab for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, China; J.-M. Chen, C. Gao, Q. Shi, B. Shan, Yan-Jun Lei, C.-F. Dong, R. An, G.-R. Wang, B.-Y. Zhang, J. Han, China CDC, State Key Lab for Infectious Dis Prevent & Control, China
SP englisch
PO Schottland