NR AXKR
AU Dabaghian,R.
TI Detection of Proteinase K Resistant Prion Protein in Human Urine
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.62
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Recent concern about the possible secondary spread of vCJD through blood transfusion and blood products has highlighted the need for a sensitive test for the identification of PrPTSE in clinical specimens collected in a non-invasive way. In addition, a more accurate estimate of the prevalence of pre-clinical vCJD in the population may be possible if there were a screening test that could be applied to easily available material such as urine. As a step towards this goal, the detection of putative PrPTSE in the urine of CJD patients has been improved, based on proteinase K digestion of samples and Western blotting. In its present form, the test uses concentrated urine as a starting material. After proteolytic treatment followed by electrophoresis and Western blotting, membranes are incubated with an anti-PrP antibody conjugated directly with horseradish peroxidase. The presence of PrP on the membrane is indicated by a colorimetric detection system. The results of this study demonstrate the presence of proteinase K resistant bands in urine from some sCJD and vCJD patients, but not in unaffected individuals.
AD R. Dabaghian, Health Protection Agency - Center for Infections, UK
SP englisch
PO Schottland