NR AXKQ
AU Costa,C.; Vidal,E.; Domenech,A.; Pumarola,M.; Bassols,A.; Rauch,U.
TI Central Nervous System Extracellular Matrix Changes in Scrapie Infected Mice
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.66
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
The mature extracellular matrix (ECM) of the central nervous system mainly contains hyaluronic acid, chondroitin sulphate proteoglycans including the lectican family, and glycoproteins such as tenascin-R. They are either dispersed in the neuropil or forming aggregates around neurons that are known as perineuronal nets (PNNs). In several neurodegenerative diseases it has been observed that remodelling of the ECM implies the modification of its components. In human transmissible spongiform encephalopathies (TSEs) loss of PNNs related to PrPres deposition has been reported. Scrapie is a neurodegenerative disease belonging to the group of TSEs that affects small ruminants (sheep and goat).
We have histochemically evaluated the alterations of the PNNs and their components, tenascin-R, brevican, neurocan (two lecticans) and hyaluronic acid, in a murine model of scrapie. C57BL/6 mice were inoculated intraperitoneally with the Rocky Mountain Laboratory (RML) Scrapie strain. Euthanasia was performed at 260 dpi, when evident clinical signs of the disease were manifest. A thorough neuropathological characterisation was performed by assessing histopathological changes, PrPres deposition and astroglial and microglial reaction. ECM changes were studied parallel to GFAP immunostaining, as an indicator of the degree of lesion.
A dramatic loss of PNNs was observed, as well as of its components: tenascin-R, brevican and, to a lesser extent of hyaluronic acid. On the contrary, neurocan was increased in the neuropil. All these changes were observed in areas where GFAP immunostaining showed more reactive astrocytes, suggesting that the most relevant ECM alterations happen in areas with the major degree of astrogliosis.
This study was financed by the project EET2002-05168-C04 of the Spanish Ministry of Science and Technology (MCyT).
AD C. Costa, A. Domènech, Veterinary Faculty, UAB, Spain; E. Vidal, CReSA, Priocat Laboratory, Spain; M. Pumarola, Animal Medicine and Surgery Department, Spain; A. Bassols, Veterinary Faculty, UAB, Spain; U. Rauch, Institute of Clinical Sciences, Lund University, Sweden
SP englisch
PO Schottland
EA pdf-Datei und Poster (Posterautoren um A. Domenech reduziert)