NR AXKD
AU Cancellotti,E.; Hennion,R.; Plinston,C.; Manson,J.C.; Barron,R.M.
TI Investigation of Cellular Tropism and Spreading Mechanisms of Different Scrapie Strains in Tissue Cultures
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.145
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
In transmissible spongiform encephalopathies, neurons are supposed to be the main target for different strains of the infectious agent. However it is possible that some strains may replicate in different cell types before spreading into neurons. To investigate this hypothesis, we have used a non neuronal cell line. These cells responded differently to infection with a number of scrapie strains suggesting that strains may have different cellular tropism for different cells of the brain. Surprisingly, persistently infected cells were then able to kill wild type or PrP knock-out primary neurons in co-culture showing how some strains may spread between different cells with PrP independent mechanisms. Moreover we are also using different co-culturing systems between persistently infected non neuronal cells and primary neurons to investigate the mechanisms by which different strains can cause neurodegeneration.
The data presented here may provide new information about TSE infectious process showing how different TSE strains can have alternative mechanisms to infect cells and cause neurodegenaration. These results may also be important for future therapeutic and diagnostic approaches for this kind of infectious diseases.
AD E. Cancellotti, University of Padova, Italy; R. Hennion, Institute for Animal Health, UK; C. Plinston, J.C. Manson, R.M. Barron, Roslin Institute, Neuropathogenesis Unit, UK
SP englisch
PO Schottland
EA pdf-Datei und Poster (Posterautoren: E. Cancellotti, C. Plinston, R. Hennion, E. Sartori, J.C. Manson und R.M Barron)