NR AXJZ
AU Bruederle,C.E.; Hnasko,R.; Garcia,R.A.; Haas,M.J.; Kraemer,T.; Marmer,W.N.; Carter,J.M.
TI Decontamination of MBM Infected with Pathogenic Prion Proteins Using a Biodiesel Process
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.112
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
The recent epidemic of bovine spongiform encephalopathy (BSE) in cattle is linked to a subsequent surge in incidence of variant Creutzfeldt-Jakob Disease, a transmissible spongiform encephalopathy (TSE) affecting humans. This has led to world-wide drop in the market for beef by-products, such as meat-and-bone meal (MBM) a fatcontaining product traditionally used as an animal feed. Our study evaluated a method for producing biodiesel from MBM for decontamination of TSE. We predict that, in addition to producing valuable fuel, the method also destroys any TSE infectivity present, while simultaneously producing a potentially value-added solid residue. As a model for a rendered TSE-infected animal carcass we spiked MBM with scrapie infected hamster brain. Laboratory scale conditions produced fatty acid methyl esters (for use as a biodiesel) using alkaline methanolysis, through incubation of MBM with
0.25 M sodium methoxide at 35C for 2 hours with vigorous shaking. We evaluated all 3 resulting phases (biodiesel, glycerol, and solids) for residual scrapie TSE in vitro and in vivo. Western blot results indicate the protein associated with TSE infectivity, PrPsc, is completely destroyed under these alkaline methanolysis conditions. Definitive in vivo tests, involving intra cranial inoculation of hamsters, are pending incubation.
AD C.E. Bruederle, R. Hnasko, J.M. Carter, US Dept Agriculture, Foodborne Contaminants Research Unit, USA; R.A. Garcia, M.J. Haas, W.N. Marmer, US Dept Agriculture, Fats, Oils and Animal Coproducts Research Unit, USA; T. Kraemer, Institute of Legal Medicine, Saarland University, Forensic Toxicology, Germany
SP englisch
PO Schottland