NR AXIU
AU Barnard,G.; Hopkins,L.; Seilly,D.; McConnell,I.
TI Direct Detection of Disease-Associated Prions in Brain and Lymphoid Tissue Using Antibodies Recognising The Extreme N-Terminus of PrPc
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.172
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB A simple diagnostic test is described for the detection of TSE in bovine, ovine and human brain and lymphoid tissue that obviates the use of proteinase K as a discriminating reagent. The immunoassay utilises high affinity anti-peptide antibodies that appear blind to the normal isoform of prion protein (PrPc). These reagents have been produced with novel N-terminal chimeric peptides and we hypothesise that the retention and stability of the extreme N-terminus of PrP in the disease-associated aggregate makes it an operationally specific marker for TSE. Accordingly, the assay involves a simple one-step capture of PrPsc followed by detection with a europiumlabelled anti-PrPc antibody after homogenisation of the tissue directly in 8M-guanidine hydrochloride. This rapid assay clearly differentiates between levels of diseaseassociated PrP extracted from brain and lymphoid tissues taken from histologyconfirmed TSE positive and negative cattle and sheep. In being PK independent, this assay has application to the detection of PrPsc in atypical scrapie where the diseaseassociated prion is more susceptible to proteolysis.
AD G. Barnard, L. Hopkins, D. Seilly, I. McConnell, University of Cambridge, Department of Veterinary Medicine, UK
SP englisch
PO Schottland
EA pdf-Datei und Poster (Posterautoren ergänzt um S. Moorthie und P. Tonks)