NR AXIN
AU Baier,M.; Riemer,C.; Bamme,T.; Gültner,S.; Heise,I.; Mok,S.W.F.; Klemm,U.
TI Chemokine CXCL13 Expression in Scrapie-Infected Brain Tissue
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.58
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB The gliosis in scrapie-infected brain tissue is associated with an upregulation of cytokines and chemokines. One of the highly induced chemokines is CXCL13, in the periphery known to be a chemoattractant for B-cells. CXCL13 mRNA-levels are upregulated at early stages of the disease and show a further increase during disease progression. Immunohistochemical time-course studies confirmed increasing CXCL13 expression in the CNS during scrapie infection. Using a double-staining procedure with anti-GFAP and anti-CXCL13 antibodies we could show for the first time that CXCL13 is expressed by activated astrocytes in the CNS. To gain more insights into the functional biology of this chemokine in the CNS mice transgenic for CXCL13 were generated. To ensure astrocyte-specific CXCL13-expression, a GFAP promoter construct was used. CXCL13 transgenic mice showed a normal development, fertility, and morphology. To investigate the role of this chemokine in the pathogenesis of neurodegenerative amyloidosis, 6 weeks-old transgenic mice were infected intracerebrally with the scrapie strain 139A and characterized in comparison to similarly infected age- and sex-matched wild-type controls. Infected CXCL13 transgenic mice showed reduced survival times. Changes in gliosis and associated expression of cytokines, chemokines and other inflammatory factors will be analysed by immunohistochemistry and real-time PCR.
AD M. Baier, Robert-Koch-Institute, Germany; C. Riemer, T. Bamme, S. Gültner, I. Heise, S. Mok, Robert-Koch-Institute, Project Neurodegenerative Diseases, Germany; U. Klemm, Max Planck Institute for Infection Biology, Germany
SP englisch
PO Schottland
EA pdf-Datei und Poster (Posterautoren: I. Heise, T. Bamme, S. Gültner, S.W.F. Mok, S. Tröller, C. Riemer, U. Klemm und M. Baier; Postertitel: Transgenic overexpression of CXCL13: Accelerated disease progression)