NR AXIG
AU Aubry,F.; Urbain,R.; Prost,J.F.
TI A Powerful Cell Sorter at the Service of the Prion Research Community
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.35
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
The foundation Alliance Biosecure aims to sponsor research on the analysis,
comprehension and management of risks for public health, linked to microbiological agents, in particular prions. Recognized as a Public Utility in December 2006 by the French Government, Alliance Biosecure promotes the discovery of technological solutions aimed at improving the detection, elimination and inactivation of these agents within biological products used in human therapy.
Indeed biological safety regarding prion risk constitutes a major public health issue, as publicized by the report of a fourth human variant Creutzfeldt-Jakob Disease (vCJD) infection attributed to blood transfusion, in the UK.
For this purpose, Alliance Biosecure has launched in 2007 a first call for grant applications and acquired laboratory equipment that will be made available to selected projects. Of special note is the availability of a three-laser Influx(R) cell sorter, located in a BSL-3 containment facility (NeuroPrion CEA Research Platform, Fontenay-aux-
Roses).
This cell sorter was developed by Cytopeia (Seattle, WA, USA), with 12 detectors and a modified design suitable for Prion experiments, including specially adapted decontamination procedures.
Here, we present several different experiments that illustrate the capabilities of this cell sorter. We show its capacity to separate complex cell populations and to isolate cells (up to a rate of 50.000/s) as a function of cell surface marker expression (up to 10 markers simultaneously), Cell Cycle, and intracellular Calcium concentration.
Alliance Biosecure is proud to make available to scientific community this equipment, that will constitute a powerful tool to study & isolate rare cellular sub-populations in blood or in cell culture.
AD F. Aubry, R. Urbain, J.F. Prost, Alliance Biosecure Foundation, France
SP englisch
PO Schottland
EA pdf-Datei und Poster (Posterautoren: F. Aubry, C. Picoli, V. Nouvel, F. Mouthon und R. Urbain; Postertitel abgeschnitten)