NR AXID
AU Apostolidou,V.; Bodemer,M.; Zerr,I.; Sklaviadis,T.
TI Tissue Plasminogen Activator's Level in Cerebrospinal Fluid as a Potential Biomarker for Creutzfeldt-Jakob Disease
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.100
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Human tissue plasminogen activator (tPA) is a protease of the S1 trypsin family which converts plasminogen to active plasmin. Plasmin is important in cell migration, inflammation and tumor invasion and tPA has been reported to be involved in neurodegeneration and neuronal plasticity. Furthermore, it is found to be secreted in response to mental stress. tPA-mediated activation has been shown to be stimulated in the presence of partially denatured proteins including PrPc and it has also been shown that apo-PrP (PrP not bound to Cu2+) stimulates this system, especially the NH2-terminal fragment. Another finding regarding the relationship between PrP and plasminogen is that the later binds to the disease associated isoform of prion protein, as well as to recombinant PrP (rPrP). Previously we have shown that tPA activity and expression levels was higher in Transmissible Spongiform Encephalopathies (TSE) infected animals compared to normal ones. Here we report our data of tPA activity in Cerebrospinal Fluid (CSF) samples from several Creutzfeldt-Jakob Disease (CJD) patients. Preliminary results show that tPA activity levels are elevated in CSF from CJD patients as compared to control ones. Normally, tPA levels do not exceed the range of 1ng/ml limit and are elevated in CSF from patients affected with a variety of other diseases, including Multiple Sclerosis, Post-Haemorrhagic Ventricular Dilatation (PHVD) and Bacterial Meningitis. CSF is a potential valuable source for detection of human TSEs.
AD V. Apostolidou, T. Sklaviadis, Aristotle University of Thessaloniki, Greece; M. Bodemer, I. Zerr, National Reference Center for TSE Surveillance, Georg-August University, Germany
SP englisch
PO Schottland