NR AXHR
AU Aguib,Y.; Gilch,S.; Ertmer,A.; Schätzl,H.M.
TI Neuroendocrine Cultured Cells Resolve Transient Prion Infection by Down-Regulation of PrPc
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.147
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Cell culture models for prion diseases are mainly of neuronal origin. However, in human and animal prion diseases, the pathological isoform of the cellular prion protein PrPc, designated PrPsc, as well as prion infectivity are found in a variety of extraneural tissues. Nevertheless, prion disease pathology is restricted to the central nervous system. Therefore, it is desirable to establish non-neuronal cell culture models susceptible to prion infection in order to study prion biogenesis and phenotypic alterations related to infection. Pheochromocytoma and insulinoma cell lines are derived from neuroendocrine tumours forming the interface between endocrine and nervous system. We investigated the susceptibility of such murine cell lines to prion infection and found that they were able to transiently propagate PrPsc. Surprisingly, prion infection was abrogated by down-regulation of PrPc expression upon exposure to prion-infected material. The cell lines described here provide novel models for studying PrPc regulation upon exposure to prions.
AD Y. Aguib, S. Gilch, A. Ertmer, H.M. Schätzl, Institute of Virology, Technical University of Munich, Germany
SP englisch
PO Schottland
EA pdf-Datei und Poster (Autorenliste ergänzt um M.H. Groschup)