NR AXFK
AU Hamir,A.N.; Miller,J.M.; Kunkle,R.A.; Hall,S.M.; Richt,J.A.
TI Susceptibility of cattle to first-passage intracerebral inoculation with chronic wasting disease agent from white-tailed deer
QU Veterinary Pathology 2007 Jul; 44(4): 487-93
PT journal article
AB Fourteen, 3-month-old calves were intracerebrally inoculated with the agent of chronic wasting disease (CWD) from white-tailed deer (CWDwtd) to compare the clinical signs and neuropathologic findings with those of certain other transmissible spongiform encephalopathies (TSE, prion diseases) that have been shown to be experimentally transmissible to cattle (sheep scrapie, CWD of mule deer [CWDmd], bovine spongiform encephalopathy [BSE], and transmissible mink encephalopathy). Two uninoculated calves served as controls. Within 26 months postinoculation (MPI), 12 inoculated calves had lost considerable weight and eventually became recumbent. Of the 12 inoculated calves, 11 (92%) developed clinical signs. Although spongiform encephalopathy (SE) was not observed, abnormal prion protein (PrPd) was detected by immunohistochemistry (IHC) and Western blot (WB) in central nervous system tissues. The absence of SE with presence of PrPd has also been observed when other TSE agents (scrapie and CWDmd) were similarly inoculated into cattle. The IHC and WB findings suggest that the diagnostic techniques currently used to confirm BSE would detect CWDwtd in cattle, should it occur naturally. Also, the absence of SE and a distinctive IHC pattern of CWDwtd and CWDmd in cattle suggests that it should be possible to distinguish these conditions from other TSEs that have been experimentally transmitted to cattle.
IN Hamir et al. inokulierten 3 Monate alte Kälber intrazerebral mit Weißwedelhirsch-Hirnhomogenat. Von 12 Kälbern erkrankten 11 und erwiesen sich immunhistochemisch sowie per Western blot als TSE-positiv, obwohl genau wie bei mit Scrapie oder Maultierhirsch-CWD infizierten Rindern keine spongiforme Degeneration beobachtet wurde.
MH Animals; Blotting, Western; Brain/*metabolism/pathology; Cattle; Cattle Diseases/pathology/*transmission; Deer/*metabolism; Disease Susceptibility; Immunohistochemistry; Male; Serial Passage; Wasting Disease, Chronic/pathology/*transmission
AD National Animal Disease Center, ARS, USDA, Ames, IA 50010, USA. ahamir@nadc.ars.usda.gov
SP englisch
PO USA