NR AXBI
AU Cooper,J.K.; Ladhani,K.; Minor,P.D.
TI Reference materials for the evaluation of pre-mortem variant Creutzfeldt-Jakob disease diagnostic assays
QU Vox Sanguinis 2007 May; 92(4): 302-10
PT comparative study; evaluation studies; journal article; research support, non-u.s. gov't
AB BACKGROUND AND OBJECTIVES: A standard panel of materials is needed for the evaluation of assays being developed for the diagnosis of variant Creutzfeldt-Jakob disease. MATERIALS AND METHODS: Tissues from human and animals incubating transmissible spongiform encephalopathy disease have been prepared, aliquoted and where possible characterized by in vitro methods. RESULTS: A standardized preparation of materials has been generated. CONCLUSIONS: Large-scale preparations of tissues and blood fractions can be used to directly compare the sensitivities of assays using different formats.
MH Animals; Base Sequence; Blotting, Western/methods/standards; Brain Chemistry; Creutzfeldt-Jakob Syndrome/blood/*diagnosis/genetics; DNA/genetics; Female; Humans; Immunoassay/methods/standards; PrPsc Proteins/blood/genetics/isolation & purification/standards; Prions/blood/genetics/*isolation & purification/standards; Protein Conformation; Reference Standards; Scrapie/blood/diagnosis/genetics; Sheep; Spleen/chemistry; Tissue Distribution
AD CJD Resource Centre, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK. jcooper@nibsc.ac.uk
SP englisch
PO England