NR AWTG
AU Arsac,J.N.; Andreoletti,O.; Bilheude,J.M.; Lacroux,C.; Benestad,S.L.; Baron,T.G.M.
TI Similar biochemical signatures and prion protein genotypes in atypical scrapie and Nor98 cases, France and Norway
QU Emerging Infectious Diseases 2007 Jan; 13(1): 58-65
IA http://www.cdc.gov/ncidod/eid/13/1/58.htm
PT journal article; research support, non-u.s. gov't
AB Isolates of atypical scrapie recently identified in sheep and goats in France were compared with Nor98 isolates reported in Norway. Western blot methods for characterization of the protease-resistant prion protein showed that all these isolates shared a unique biochemical signature: 5 groups of bands, including a characteristic band of apparent low molecular weight (11 kDa). This pattern could originate from the presence of 3 different protease cleavage products, including the 11 kDa most likely cleaved at both N- and C-sides of the protein. Genetic data, which strongly suggested the higher susceptibility of AHQ and AF141RQ animals in French cases, resembled earlier data from Nor98 scrapie.
MH Alleles; Animals; Brain Stem/metabolism; France/epidemiology; Genotype; Goat Diseases/epidemiology; Goats/metabolism; Norway/epidemiology; Polymorphism, Genetic; Prions/*genetics/*isolation & purification; Scrapie/*epidemiology/metabolism; Sheep/metabolism
AD Jean-Noël Arsac, Thierry Baron, Agence Française de Sécurité Sanitaire des Aliments, Lyon, France; Olivier Andreoletti, Caroline Lacroux, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France; Jean-Marc Bilheude, Bio-Rad, Marnes-la-Coquette, France; Sylvie L. Benestad, National Veterinary Institute, Oslo, Norway
SP englisch
PO USA