NR AWQW
AU Yim,I.; Woo,H.J.
TI Real-time immuno-PCR for prion detection in bovine meat
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions
PT Konferenz-Poster
AB Prion diseases are fatal neurodegenerative diseases caused by conformational change of prion protein (PrP). Recent reports have shown that PrP accumulates in the skeletal muscles of diseased animals and man. As the Western blot or ELISA, officially used to detect PrP, are not sufficient because of very low level of PrP in the skeletal muscles of animals, the novel approaches to improve current protocols for detecting PrP in meat. As a model system for bovine meat, we have expressed and purified recombinant bovine (recBo) PrP, and chosen several peptides from PrP of murine and bovine for immunizing goat to get polyclonal antibodies. Using recBo PrP and polyclonal antibodies,, immuno-polymerase chain reaction (IPCR) was performed to improve the limit of detection by ELISA. The IPCR products were quantitatively analyzed in real-time. Sensitivity of real-time IPCR was 5 fg/ml that could be applied in meat. The concentration of PrPc in meat homogenate was calculated as 3-80 ng/ml on our experimental conditions.
AD Inbeen Yim, Laboratory of Immunology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742 Korea; Hee-Jong Woo, Institute for Zoonotic Diseases, College of Veterinary Medicine, Seoul National University, Seoul, 151-742 Korea
SP englisch
PO Italien