NR AWQI
AU Weissmann,C.; Mahal,S.P.; Baker,C.A.; Demczyk,C.
TI Application of the scrapie cell assay (SCA) to discriminate between different mouse prion strains
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Oral sessions ORAL-36
PT Konferenz-Vortrag
AB The scrapie cell assay (SCA; Kloehn et al., PNAS 100, 11666-71 (2003)) is a rapid, cell-based method for the quantification of certain mouse prion strains. With a newly isolated cell line we can now complete the assay within 10 days and detect prions quantitatively down to a 10-8 dilution of RML scrapie brain homogenate. In addition, we have developed cell lines that show different susceptibilities to different murine scrapie prion strains. With a panel of four cell lines we can discriminate between at least three prion strains within three weeks, a task that would take a year or more with the classical mouse assay. Interestingly, from one parental cell line we have isolated sublines that show very different relative susceptibilities to two prion strains. This raises the question not only as to how cells recognize prion strains but also as to how the variability of this property comes about.
AD Department of Infectology, Scripps Florida, Jupiter FL 33458, USA
SP englisch
PO Italien