NR AWOK
AU Steele,P.; Gonzalez,L.; Finlayson,J.; Kennedy,D.; Hamilton,S.; Eaton,S.; Rodger,S.; Reid,H.W.; Chianini,F.; Dagleish,M.P.
TI Advantages of the Moredun RecspecTM for TSE diagnosis in ruminants
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-50
PT Konferenz-Poster
AB Diagnosis of ruminant TSE disease currently relies on detection of abnormal PrP in tissue samples. While largely confined to the CNS in BSE of cattle, abnormal PrP accumulates in tissues of the lymphoreticular system in sheep scrapie and CWD-infected deer. Abnormal PrP can often be detected in LRS tissues before any CNS involvement, and in otherwise asymptomatic animals. A biopsy of LRS tissue in the live animal is therefore a useful test of infection status. Previous studies have focused on biopsies of palatine tonsil and third eyelid; here we report on the development of a biopsy technique for rectoanal mucosa-associated lymphoid tissue (RAMALT). We have designed the Moredun RecSpecTM, a speculum which allows observation and accurate sampling of the rectal mucosa, specifically targeting the lymphoid follicle-rich area between mucosal folds. We employed this method to take sequential biopsies from sheep of the Moredun natural scrapie infected Suffolk flock. Biopsy samples were fixed in buffered formalin and assessed by immunohistochemistry for the number of follicles (valid biopsy if n >=5) and the presence of abnormal PrP. RAMALT biopsies taken using the Moredun RecSpecTM gave better quality biopsy samples (97% biopsies >5 follicles) compared with a parallel palatine tonsil biopsy (75% biopsies >5 follicles) with a significantly greater average number of follicles. Animals do not require sedation and exhibit no signs of distress or subsequent complications allowing sequential biopsies from the same animal. With experience the technique is very rapid (<1min/animal) and the equipment is inexpensive (~ GB£1.50/animal). Sample size is adequate for detection of abnormal PrP in ELISA systems, and we have successfully adapted the technique for use in red deer. RAMALT biopsy by this method can be a useful tool in the detection of preclinical and clinical disease in experimental studies or expanded to flock testing as part of disease control strategy.
AD P. Steele, J. Finlayson, D. Kennedy, S. Hamilton, S. Eaton, S. Rodger, H.W. Reid, F. Chianini, M.P. Dagleish: Moredun Research Institute, Edinburgh, UK; L. González: Veterinary Laboratory Agency, Lasswade, Edinburgh, UK. E-mail: philip.steele@moredun.ac.uk
SP englisch
PO Italien