NR AWLZ
AU Pilon,J.L.; Loiacono,C.M.; Okeson,D.; Thomsen,B.V.; Rhyan,J.; VerCauteren,K.; Miller,L.A.
TI Early successes in the development of a PrPsc vaccine using a mouse model system
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions THE-12
PT Konferenz-Poster
AB The United States National Wildlife Research Center (NWRC) develops effective wildlife damage management methods through research to understand human and wildlife conflicts. As part of our mission, the NWRC has a very active Chronic Wasting Disease Project that has several ongoing research areas that aim to understand and prevent the spread of the endemic prion disease of cervids, Chronic Wasting Disease (CWD), in North America. Building on the success of the NWRC's vaccine based immuno-contraceptive efforts, we are determining the feasibility of developing a vaccine to reduce or prevent the spread of CWD in domestic and wild populations of cervids. Using a unique adjuvant technology developed at the NWRC, we tested five prion peptides coupled to a carrier protein to identify possible CWD vaccine candidates. Following vaccine treatments which produced antibodies to the individual peptides as measured by ELISA in CL57BL/10 mice, all mice were challenged with the Rocky Mountain Laboratory mouse scrapie strain via an intraperitoneal route. The progression of the disease was monitored by western-blot analysis and IHC. All five peptides tested have increased average days until death relative to the challenge controls. Two peptides increased survival very significantly (p = 0.0005). The unique nature of the adjuvant and the use of a relatively new carrier protein may have provided a basis for these promising initial results. While vaccinate groups still succumb to the disease, results suggest that an immunological approach to controlling CWD or other prion diseases is plausible. Our future directions are to continue work in a mouse model system to improve vaccine constructs and formulations in addition to moving into more relevant animals such as Ovis aries and/or cervids.
AD J.L. Pilon, J. Rhyan, K. VerCauteren, L.A. Miller: USDA/APHIS/WS National Wildlife Research Center, 4101 LaPorte Ave, Fort Collins CO, 80521 USA; C.M. Loiacono, B.V. Thomsen: National Veterinary Services Laboratories 1800 Dayton Ave Ames, IA 50010, USA; D. Okeson: San Antonio Zoo 3903 N. St. Mary's Street San Antonio, TX 78212-7183 USA. E-mail: john.l.pilon@aphis.usda.gov
SP englisch
PO Italien