NR AWLH
AU O'Rourke,K.I.; Herrmann,L.M.
TI The role of prenatal and postnatal ovine chimerism in scrapie control programs
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions GEN-20
PT Konferenz-Poster
AB Naturally occurring chimerism in mammalian species includes prenatal exchange of stem cells between fetuses due to anastomoses of placental vasculature, transfer of fetal cells to the maternal bloodstream during pregnancy, and transfer of maternal cells to progeny via milk or colostrum. We have demonstrated that exchange of cells between lambs sharing a uterine environment results in accumulation of PrP-d in placental tissues with Prnp genotypes generally considered resistant to classical scrapie. The objective of this study was to examine the frequency of placental chimerism in multiple births and the effect of prenatal chimerism on genetic analysis of lambs. Further, we examined the role of fetal cells in the maternal circulation of pluripotent ewes as a potential source of Prnp genotyping error. Methods included allele-specific polymerase chain reaction assay of Prnp, MHC II DRB1 typing, and microsatellite analysis. Relative sensitivity of the methods, age of the animals, and number of pregnancies were considered as contributing factors when evaluating the methods for accuracy and cost efficiency. Sensitive and specific methods for identifying the true Prnp genotype of sheep is an important component of the US scrapie control program.
AD US Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Pullman, WA, USA. E-mail: korourke@vetmed.wsu.edu
SP englisch
PO Italien