NR AWKW
AU Nappi,R.; Meloni,D.; Gai,F.; Gastaldi,D.; Martucci,F.; Acutis,P.L.; Maurella,C.; Caramelli,M.; Bozzetta,E.
TI Evaluation of in field rapid tests performance for the diagnosis of ovine TSE
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-39
PT Konferenz-Poster
AB Scrapie is known in Italy since 1995 and the number of outbreaks has been dramatically increasing since the introduction of active surveillance in small ruminants in 2002, by means of rapid tests already approved for BSE. We performed a comparative evaluation among the 2 rapid tests applied in Italy for both sheep and bovine TSE active surveillance (Bio-Rad TeSeE and Enfer TSE kit version 2.0, Abbot) and the IDEXX HerdChek Scrapie Antigen kit and Bio-Rad TeSeE Sheep/Goat, recommended from the EFSA evaluation for classical and atypical Scrapie detection form the obex region, in order to verify their performance in field for the detection of ovine TSE. The caudal brain stems of Biellese sheep with various genotype belonging to a Piedmont flock with high scrapie prevalence were collected. Samples were subjected to a pre-homogenization protocol and split in aliquots for each test and for the NaPTA western-blot to confirm any positive rapid tests result. IDEXX HerdChek Scrapie Antigen kit and Bio-rad TeSeE sheep and goat could detect additional positive cases in comparison with the two other systems, stressing the importance of applying the most sensitive rapid methods of screening to strengthen TSE active surveillance
AD Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle D'Aosta, CEA, Turin, Italy. E-mail: cea.validazioni@izsto.it
SP englisch
PO Italien