NR AWJU
AU Matucci,F.; Mazza,M.; Nodari,S.; Pagano,M.; Peletto,S.; Nappi,R.; Meloni,D.; Corona,C.; Iulini,B.; Davico,P.; Casalone,C.; Bozzetta,E.; Caramelli,M.; Acutis,P.L.
TI Scrapie in sheep carrying the lysine 171-allele of the prion protein gene
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-35
PT Konferenz-Poster
AB Susceptibility to scrapie in sheep is determined by the infective strain and the host PrP genotype, deriving from amino acidic changes encoding five alleles: A136R154Q171, VRQ, AHQ, ARH, ARR. In addition to these polymorphisms, a fourth amino acidic change (glutamine to lysine) has been described at codon 171, generating the ARK allele. For this allele no data about scrapie susceptibility are currently available, because of its rarity in sheep population. To investigate the scrapie susceptibility linked to this allele, eight ARK/--- animals coming from a Piedmontese outbreak and 20 ARK/--- sheep from an outbreak in Lombardia Region, were analysed by a SAF immunoblotting, by immunohistochemistry and by two different rapid tests. Three positive ARK/--- sheep, coming from the first scrapie outbreak, were found. Although our results do not permit to estimate the level of susceptibility associated to this allele yet, these findings show that the animals carrying the ARK allele of the PrP gene are not fully resistant to scrapie.
AD F. Martucci, M. Mazza, S. Nodari, M. Pagano, S. Peletto, R. Nappi, D. Meloni, C. Corona, B. Julini, C. Casalone, E. Bozzetta, M. Caramelli, P.L. Acutis: CEA - Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Turin, Italy; P. Davico: Presidio Multizonale di Profilassi e Polizia Veterinaria - ASL 4, Turin, Italy. E-mail: francesca.martucci@izsto.it
SP englisch
PO Italien