NR AWJE
AU Lopez,C.; Hurtado,D.; Cantero,L.; Anadon,E.; Gomez-Tejedor,C.; Mayoral,T.
TI Frequency of scrapie resistance associated allele Lisine-171 in Spanish ovine breeds
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions EPI-13
PT Konferenz-Poster
AB Spanish ovine genotyping programme is coordinated by Ministry of Agriculture, Fisheries and Food and is carried out by one National Reference Laboratory (Laboratorio Central de Veterinaria) (R.D. 1312/2005) and 6 authorised laboratories. Since the beginning, more than 1.000.000 animals has been analysed from 40 ovine pure breeds. At LCV SNaPshot technology is used to detect polymorphisms located at codons 136, 154 and 171 of prnp gene. Five most common alleles were VRQ, ARQ, ARR, AHQ and ARH, nevertheless the ARK allele was also found. For that purpose an improved method has been designed for Scrapie genotyping and ARK detection by microsequencing. More than 2000 animals belonging to 15 pure breeds show at least one allele ARK, all of then are health animals included in the National Genotyping Programme. No ARK alleles have been found in Scrapie affected cases. Most of animals are from breed Ojinegra de Teruel (39.2%), Assaf (12.3%), Rasa Aragonesa (16.1%) and Navarra (12.3%). Frequencies of polymorphism Lysine-171 are less than 1% in the others 13 breeds and near 7% in two of them. Results of ARK incidence in Spanish ovine breeds and distribution will show and data concerning to resistance associated alleles in ARK carrying animals.
AD Laboratorio Central de Veterinaria. National Reference Laboratory for ovine prnp gene analysis. MAPA. Algete, Madrid, Spain. E-mail: tmayoral@mapya.es
SP englisch
PO Italien