NR AWGX
AU Herrero Hernandez,E.; Casalone,C.; Bozzetta,E.; Iulini,B.; Martucci,F.; Manea,B.; Davari,A.; Ghiglione,A.; Spinelli,P.; Bergo,V.; Pira,E.; Caramelli,M.
TI Exploring the metal-triggered hypothesis in scrapie and BSE
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-25
PT Konferenz-Poster
AB Metal imbalances may trigger prion diseases. We measured selected metals in biological samples from healthy and prion-affected sheep and cows to detect possible differences supporting this hypothesis. Mn, Al, Cu, Zn and Se levels were quantified by atomic absorption spectrophotometry (Perkin Elmer 4100 and 5100) on blood/serum and CNS areas from animals from scrapie and BSE foci. Frozen tissues provided by IZS Turin after decontamination with sodium hypochlorite (2% chlorine) followed by autoclaving (134° for 1h) underwent de-freezing at room temperature for 24 hours followed by dry thermal treatment (80° for 2 h). After weighing and hydrolysis (48 h in nitric acid 60%, 0.5 ml, Ultrapur), bi-distilled water (4.5 ml) was added and the samples were kept in this solution for further 24 hours. Results were quoted in g/g dry weight. Blood/serum samples were analysed after 1:10 dilution. We analysed 64 SNC samples from 6 healthy sheep, 41 SNC samples from 5 scrapie-affected sheep, 14 SNC samples from 1 healthy cow, 60 SNC samples from 5 BSE-affected cows (see poster data); Blood (b)/serum (s) samples: 110 ovine (108 healthy=H, 2 scrapie=Sc) and 9 healthy bovine samples. Metal blood-serum levels were as follows (arithmetic mean ± standard deviation; geometric mean): Mn-b (µg/l): H: 11.28 ± 7.04; 9.14 Sc: 11.15 ± 2,47; 11.01. Fe-b (mg/l): H: 386 ± 51; 382 Sc: 360 ± 39; 358. Cu-s (mg/l): H: 0.87 ± 0.32; 0.77 Sc: 1.01 ± 0.04; 1.01.Zn-s (mg/l):H: 1.10 ± 0.33; 1.05 Sc: 1.0 ± 0.47; 1.04. Al-s (µg/l): H: 9.13 ± 8.83; 7.45 Sc: 9.90 ± 2.55; 9.73. The study is ongoing to achieve a greater number of samples and thus provide significant results. To date no evident differences in metal levels are observed. Acknowledgements: Italian Ministry of Health for research grant.
AD E. Herrero Hernandez, A. Davari, A. Ghiglione, P. Spinelli, V. Bergo, E. Pira: Traumatology, Orthopaedics and Occupational Health Department, University of Turin, Italy; C. Casalone, E. Bozzetta, B. Iulini, F. Martucci, B. Manea, M. Caramelli: CEA, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Turin, Italy. E-mail: elena.herrero@unito.it
SP englisch
PO Italien