NR AWGG
AU Gyllberg,H.; Almeida,A.; Bedecs,K.
TI Does presence of PrPsc in lipid rafts affect SRC family kinase signaling?
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions CE-17
PT Konferenz-Poster
AB Since the conversion of PrPc to PrPsc have been shown to occur in lipid rafts and that PrPc might act as a signaling protein we hypothesize that misfolded PrP can cluster PrPc together and initiate an uncontrolled signaling of intracellular signaling proteins e.g. the Src family kinase Fyn. Lipid rafts are also known as detergent resistant membranes i.e. they are resistant to certain nonionic detergents at low temperatures. This property is used to isolate lipid rafts as lipid raft complexes float to low density during sucrose gradient centrifugation. These fractions are further analyzed by western blot - immunoblotted with antibodies against Fyn and active Fyn. In vitro kinsae asssays are also performed on flotation fractions. Preliminary results show an increased specific activity of Fyn kinase in scrapie-infected murine immortalized hypothalamic cells, ScGT1-1 compared to uninfected cells after immunoblotting with clone 28 (recognizes active Src and Fyn) immunoprecipitates of Fyn protein. Future studies will focus on the suggested colocalization of PrPc/PrPsc and Fyn kinase in lipid rafts. Abnormal Fyn activation may be a key element in the neuropathology in prion disease.
AD Department of Biochemistry and Biophysics, Stockholm Universtiy, Svante Arrhenius väg 12, SE 106 91 Stockholm, Sweden
SP englisch
PO Italien