NR AWEJ
AU Edwards,J.; Horton,R.; Everest,S.; Terry,L.
TI PrP conformers expressed on the cell surface of PBMCS isolated from animals naturally infected with scrapie during pathogenesis of disease
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-14
PT Konferenz-Poster
AB Prior to invasion of the central nervous system, PrPsc is accumulated by follicular dendritic cells and macrophages in lymphoid tissue in the majority of scrapie infected sheep. Since peripheral blood mononuclear cells (PBMCS) ubiquitously express PrP on their cell surface, haematogenic distribution is a potential route by which PrPsc may disseminate through the lymphoreticular system and then ultimately to the CNS. Here, we have employed a panel of anti-PrP antibodies to various epitopes in attempt to distinguish between PBMCs isolated from sheep naturally infected with scrapie and age and genotype matched controls. Variations in staining profiles could imply the appearance of structural modifications of cell surface expressed PrP similar to those anticipated during PrPc to PrPsc conversion in the CNS. Briefly, PBMCs were isolated from blood samples taken from VRQ/VRQ (V/V) sheep in a high-incidence experimental scrapie flock. Cells were probed with PrP antibodies and then analysed using flow cytometry. The results were compared against age and genotype matched controls. Bloods samples were also taken from animals with scrapie and ovine BSE at the terminal stage of disease and analysed in a similar manner. Our results show statistically significant differences in antibody staining profiles between PBMCs isolated from control and scrapie
AD TSE Molecular Biology Department, Veterinary Laboratories Agency, Addlestone, Surrey, KT15 3NB, UK
SP englisch
PO Italien