NR AWBG
AU Atzori,C.; Marietti,G.; Romito,A.; Natale,G.; Taraglio,S.; Guastamacchia,G.; Orsi,L.; Imperiale,D.; Buffa,C.
TI Diagnostic value of CSF biomarkers in human prion diseases
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-03
PT Konferenz-Poster
AB Human transmissible spongiform encephalopathies (TSE), in particular Creutzfeldt-Jakob disease (CJD), are invariably fatal neurodegenerative diseases. Clinical signs and symptoms at early stage may not be easy to distinguish from other diseases by neurological findings. Diagnostic biochemical parameters, including 14-3-3 protein and tau levels in cerebrospinal fluid (CSF), have been used as diagnostic markers but these markers can also be detected in other conditions. We aimed to evaluate CSF levels of 14-3-3 protein, tau, p-tau and Ab42 as diagnostic biomarkers of human TSE in a clinical settting. CSF samples from 47 patients with a definite diagnosis of TSE and 155 non-TSE controls were analyzed. 14-3-3 protein was detected by Western blot while CSF levels of tau, p-tau and Ab42 were determined by commercially available ELISA kits. A positive CSF 14-3-3 protein was detected in 44/47 TSE patients versus 11/109 non-TSE controls (sensibility 0.94; specificity 0.90; positive predicting value 0.80; negative predicting value 0.97). Tau levels and p-tau levels (mean±SE, pg/ml) resulted significantly higher in TSE (respectively 5268.9±890.7 and 37.1±8.5 versus 507.5±41.6 and 19.2±2.0) compared non-TSE subjects (p<0.01) while there were no differences in Ab42 levels (340.0±35.6 versus 250.6±79.2, p=0.99). The detection of 14-3-3 protein in CSF and the measurement of tau and p-tau levels confirm to be valuable biomarkers in diagnostic work-up of human prion diseases in vitam.
AD C. Atzori, G. Marietti, A. Romito, G. Natale, S. Taraglio, G. Guastamacchia, D. Imperiale, C. Buffa: Centro Diagnosi Osservazione Malattie Prioniche D.O.M.P. - A.S.L. 3 Torino, Italy; L. Orsi: Dipartimento di Neuroscienze - Università di Torino Address: Ospedale Maria Vittoria Via Cibrario 72 - 10144 Torino, Italy. E-mail: domp@asl3.to.it
SP englisch
PO Italien