NR AWBB
AU Angers,R.C.; Browning,S.R.; Seward,T.S.; Sigurdson,C.J.; Miller,M.W.; Hoover,E.A.; Telling,G.C.
TI Prions in skeletal muscle of CWD infected deer
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-03
PT Konferenz-Poster
AB The zoonotic potential of chronic wasting disease (CWD) has become a public health concern since the transmission of bovine spongiform encephalopathy (BSE) prions to humans resulting in variant Creutzfeldt-Jakob disease (vCJD). Studies in mice, sheep and humans indicated that PrPsc could be detected in the skeletal muscles. Since the most probable route of human exposure to CWD is through consumption or handling of meat from infected animals, it is important to assess whether skeletal muscle from affected cervids harbors prions. CWD-susceptible Tg(CerPrP) mice were intracranially inoculated with brain and matched skeletal muscle homogenates from moribund as well as non-infected control deer. Tg mice inoculated with either brain or muscle homogenates from CWD-infected deer developed clinical illness with characteristic prion disease symptoms and the brains of recipients accumulated cervid PrPsc. The mean incubation times for animals inoculated with brain material ranged between 231 and 283 days, whereas mice receiving muscle tissue had average incubation periods between 360 and 492 days. Tg mice inoculated with material from CWD-negative deer did not develop prion disease or accumulate PrPsc. Brain and muscle samples used to inoculate Tg(CerPrP) mice were analyzed for the presence of PrPsc. Brain samples producing the shortest incubation times had levels of PrPsc detectable by Western blotting in 25 g total protein, whereas PrPsc was detectable only after sodium phosphotungstate (NaPTA) precipitation of 0.5 mg for isolates with the longest incubation periods. No protease-resistant material was detected in muscle when 50 mg total protein was precipitated with NaPTA and analyzed by Western blot. Although a possible role of prion strain variability cannot currently be dismissed, these results suggest variable prion titers in the CNS and skeletal muscle from different CWD-infected deer in the same phase of disease.
AD R.C. Angers: Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY 40536; S.R. Browning: Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY 40536, present address: Department of Infectology, Scripps Research Institute, 5353 Parkside Drive, RF-2, Jupiter, Florida, 33458; T.S. Seward: Sanders Brown Center on Aging, University of Kentucky, 3Department of Neurology, University of Kentucky; C.J. Sigurdson: Department of Microbiology, Immunology and Pathology, Colorado State University, present address: Institute of Neuropathology, University of Zürich, Schmelzbergstr 12, 8091 Zürich, Switzerland; E.A. Hoover: Department of Microbiology, Immunology and Pathology, Colorado State University; M.W. Miller: Colorado Division of Wildlife, Wildlife Research Center, Fort Collins, CO 80526; G.C. Telling: Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY 40536, and Sanders Brown Center on Aging, University of Kentucky, KY, USA. E-mail: gtell2@ uky.edu
SP englisch
PO Italien