NR AWAU
AU Acutis,P.L.; Vaccari,G.; Colussi,S.; Zuccon,F.; Lorenzetti,R.; Barbaro,K.; Marconi,P.; Eleni,C.; Casalinuovo,F.; Biagetti,M.; Santagada,G.; Agrimi,U.; Nonno,R.; Ru,G.; Maurella,C.; Caramelli,M.
TI Genetic susceptibility of Italian sheep and goats to NOR98: a case-control study
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions GEN-03
PT Konferenz-Poster
AB Prion protein (PrP) gene alleles AHQ and AF141RQ are associated with the highest risk of acquiring the atypical scrapie strain Nor98. Furthermore, Nor98 is also found in animals carrying the ARR allele, associated with resistance to classical scrapie. In Italy, until May 2006, 15 Nor98, have been detected, four of which involving goats. A case-control study has been carried out to investigate the genetic susceptibility to Nor98 in Italian sheep and goats, considering the alleles formed by polymorphisms at codons 136, 141, 154 and 171. In sheep, allele frequencies were compared, with a Chi-square-test, between 3 cases and 1301 Nor98 negative flockmates. In addition, a Chi-square comparison has been done between allele frequencies of all the 11 ovine cases found in Italy so far and allele frequencies of the Italian ovine population, coming from the genotype analysis of the sample foreseen by Reg. 999/2001 (EC), Annex III, point 7.2. In goats, 3 cases and 139 negative herdmates have been examined. In sheep a significant association of AHQ and AF141RQ with Nor98 was found (2=6.84, pvalue=0.009, OR=4.96; 2=31.21, p-value=0.000, OR=47.69, respectively). The ARR allele had no significant association, showing that neither it presents an increased risk of acquiring the disease nor it has a protective role as in classical scrapie. In goats the AHQ allele was significantly associated with Nor98 (2=4,35, p-value=0.013, OR=9.12), suggesting that genetic susceptibility in sheep and goats shares common features.
AD P.L. Acutis, S. Colussi, F. Zuccon, G. Ru, C. Maurella, M. Caramelli: Istituto Zooprofilattico del Piemonte, CEA, Turin, Italy; G. Vaccari, U. Agrimi, R. Nonno: Istituto Superiore di Sanità, Rome, Italy; R. Lorenzetti, K. Barbaro, P. Marconi, C. Eleni: Zooprofilattico del Lazio, Rome, Italy; F. Casalinuovo: Istituto Zooprofilattico del Mezzogiorno, Catanzaro, Italy; M. Biagetti: Istituto Zooprofilattico dell'Umbria, Perugia, Italy; G. Santagada: Istituto Zooprofilattico della Basilicata, Matera, Italy. E-mail: pierluigi.acutis@izsto.it
SP englisch
PO Italien