NR AWAR
AU Acin,C.; Perucchini,M.; Dalgleish,M.; Reid,H.; Pemberton,J.; Monzon,M.; Goldmann,W.
TI Polymorphisms in the protein coding region of the prion gene (Prnp) of some European and Asiatic cervid species
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions GEN-01
PT Konferenz-Poster
AB Chronic wasting disease (CWD) is a natural Prion disease which currently affects a number of cervid species in North America; these include Mule deer, White-Tailed deer, Rocky Mountain elk and Moose. Association between variation in the primary sequence of the PrP gene and disease modulation has been shown for CWD and a number of studies have analysed the genetic variability of the PrP gene in North American cervid species. European cervid species are currently considered free of this disease and the knowledge of how the PrP gene varies between and within these species is still limited. We report here the results obtained in two studies of the PRNP gene in various European deer species. Study one analysed 82 red deer (Cervus elaphus) from mainland Scotland while the second study analysed 50 red deer from a Scottish island (Isle of Rhum). Additionally, archival samples from one Axis axis, two Cervus eldii, one Dama dama and four Elaphurus davidianus were analysed. The study focussed on the open reading frame of the PrP gene. DNA was extracted from blood and analysed through direct sequencing of PCR products. We have seen amino acid polymorphisms at codons 138, 168, 208 and 226. Several silent mutations were also detected at codons 15, 21, 77, 136, 168, 172 and 217. All the animals studied were methionine homozygous at codon 132. Variation at this position has been associated with CWD modulation in Rocky Mountain elk.
AD C. Acín, M. Monzón: Department of Animal Pathology. University of Zaragoza. Zaragoza. Spain; M. Perucchini, W. Goldmann: Institute for Animal Health, Neuropathogenesis Unit, Edinburgh, UK; M. Dalgleish, H. Reid: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, UK; J. Pemberton: Institute of Evolutionary Biology, University of Edinburgh, UK. E-mail: crisacin@unizar.es
SP englisch
PO Italien