NR AVUZ
AU Sakudo,A.; Onodera,T.; Ikuta,K.
TI Prion protein gene-deficient cell lines: powerful tools for prion biology.
QU Microbiology and Immunology 2007 Jan; 51(1): 1-13
IA http://www.jstage.jst.go.jp/article/mandi/51/1/51_1/_article
PT journal article; research support, non-u.s. gov't; review
AB Prion diseases are zoonotic infectious diseases commonly transmissible among animals via prion infections with an accompanying deficiency of cellular prion protein (PrPc) and accumulation of an abnormal isoform of prion protein (PrPsc), which are observed in neurons in the event of injury and disease. To understand the role of PrPc in the neuron in health and diseases, we have established an immortalized neuronal cell line HpL3-4 from primary hippocampal cells of prion protein (PrP) gene-deficient mice by using a retroviral vector encoding Simian Virus 40 Large T antigen (SV40 LTag). The HpL3-4 cells exhibit cell-type-specific proteins for the neuronal precursor lineage. Recently, this group and other groups have established PrP-deficient cell lines from many kinds of cell types including glia, fibroblasts and neuronal cells, which will have a broad range of applications in prion biology. In this review, we focus on recently obtained information about PrP functions and possible studies on prion infections using the PrPdeficient cell lines.
ZR 84
MH Animals; *Cell Line; Cell Transformation, Viral; Hippocampus; Mice; Neurons/*chemistry/*physiology; Prions/*genetics/*physiology; Simian virus 40
AD Department of Virology, Research Institute for Microbial Diseases, Osaka University, Japan. sakudo@biken.osaka-u.ac.jp
SP englisch
PO Japan