NR AVQB
AU Chang,B.; Cheng,X.; Yin,S.; Pan,T.; Zhang,H.; Wong,P.; Kang,S.C.; Xiao,F.; Yan,H.; Li,C.; Wolfe,L.L.; Miller,M.W.; Wisniewski,T.; Greene,M.I.; Sy,M.S.
TI Test for detection of disease-associated prion aggregate in the blood of infected but asymptomatic animals
QU Clinical and Vaccine Immunology 2007 Jan; 14(1): 36-43
PT journal article; research support, n.i.h., extramural; research support, non-u.s. gov't; research support, u.s. gov't, non-p.h.s.
AB We have developed a sensitive in vitro assay for detecting disease-associated prion aggregates by combining an aggregation-specific enzyme-linked immunosorbent assay (AS-ELISA) with the fluorescent amplification catalyzed by T7 RNA polymerase technique (FACTT). The new assay, named aggregation-specific FACTT (AS-FACTT), is much more sensitive than AS-ELISA and could detect prion aggregates in the brain of mice as early as 7 days after an intraperitoneal inoculation of PrPsc. However, AS-FACTT was still unable to detect prion aggregates in blood of infected mice. To further improve the detection limit of AS-FACTT, we added an additional prion amplification step (Am) and developed a third-generation assay, termed Am-A-FACTT. Am-A-FACTT has 100% sensitivity and specificity in detecting disease-associated prion aggregates in blood of infected mice at late but still asymptomatic stages of disease. At a very early stage, Am-A-FACTT had a sensitivity of 50% and a specificity of 100%. Most importantly, Am-A-FACTT also detects prion aggregates in blood of mule deer infected with the agent causing a naturally occurring prion disease, chronic wasting disease. Application of this assay to cattle, sheep, and humans could safeguard food supplies and prevent human contagion.
MH Animals; Deer; Immunoassay; Mice; PrPsc Proteins/*analysis/chemistry/immunology; Prion Diseases/*blood/immunology/*veterinary; Prions/*blood/chemistry/*immunology
AD man-sun.sy@case.edu, Institute of Pathology School of Medicine, Case Western Reserve University, Cleveland, Room 5131, Wolstein Bldg., 2103 Cornell Rd., Cleveland, Ohio 44107-1712, USA
SP englisch
PO USA
AD School of Medicine, Case Western Reserve University, .
SP englisch
PO USA