NR AVLF
AU Lezmi,S.; Bencsik,A.A.; Baron,T.G.M.
TI PET-blot analysis contributes to BSE strain recognition in C57Bl/6 mice
QU Journal of Histochemistry and Cytochemistry 2006 Oct; 54(10): 1087-94
PT journal article
AB Identification of the strain of agent responsible for bovine spongiform encephalopathy (BSE) can be made histologically through the analysis of both distribution and intensity of brain vacuolar lesions after BSE transmission to mouse. Another useful way to distinguish the BSE agent from other prion strains is the study of the distribution of the abnormal prion protein (PrPres). For that purpose, paraffin-embedded tissue blot (PET-blot) method was applied on brains from C57Bl/6 mice infected with cattle BSE, experimental sheep BSE, or feline spongiform encephalopathy (FSE) from a cheetah. PrPres distribution was comparable, whichever of the three BSE agent sources was considered and was distinct from the PrPres distribution in C57Bl/6 mice inoculated with a French scrapie isolate or with a mouse-adapted scrapie strain (C506M3). These data confirm a common origin of infectious agent responsible for the British and French cattle BSE. They also indicate that PET-blot method appears as a precise complementary tool in prion strain studies because it offers easy and quick assessment of the PrPres mapping. Advantages and limits of the PET-blot method are discussed and compared with other established and validated methods of strain typing.
MH Acinonyx; Animals; Brain/*metabolism/pathology; Cattle; Encephalopathy, Bovine Spongiform/*metabolism/transmission; Female; Mice; Mice, Inbred C57BL; Paraffin Embedding; PrPsc Proteins/*metabolism; Research Support, Non-U.S. Gov't; Scrapie/metabolism/transmission; Sheep; Species Specificity
AD Stéphane Lezmi (s.lezmi@lyon.afssa.fr), Anna A. Bencsik (a.bencsik@lyon.afssa.fr), Thierry G. M. Baron (t.baron@lyon.afssa.fr), Unité Agents Transmissibles Non Conventionnels (ATNC), Agence Francaise de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, 31 Avenue Tony Garnier, 69364 Lyon cedex 07, France
SP englisch
PO USA