NR AVHB
AU Cartoni,C.; Schinina,M.E.; Maras,B.; Nonno,R.; Vaccari,G.; Di Bari,M.; Conte,M.; De Pascalis,A.; Principe,S.; Cardone,F.; Pocchiari,M.; Agrimi,U.
TI Quantitative profiling of the pathological prion protein allotypes in bank voles by liquid chromatography-mass spectrometry
QU Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 2007 Apr 15; 849(1-2): 302-6
PT journal article; research support, non-u.s. gov't
AB The conversion of the cellular prion protein (PrPc) into a misfolded isoform (PrP(TSE)) that accumulates in the brain of affected individuals is the key feature of transmissible spongiform encephalopaties (TSEs). Susceptibility to TSEs is influenced by polymorphisms of the prion gene suggesting that the presence of certain amino acid residues may facilitate the pathological conversion. In this work, we describe a quantitative, fast and reliable HPLC-MS method that allowed to demonstrate that in the brain of 109(Met/Ile) heterozygous bank voles infected with the mouse adapted scrapie strain 139A, there are comparable amounts of PrP(TSE) with methionine or isoleucine in position 109, suggesting that in this TSE model the two allotypes have similar rates of accumulation. This method can be easily adapted for the quantitative determination of PrP allotypes in the brain of other natural or experimental TSE models.
IN Bei hinsichtlich des Codons 109 des Prionproteins Met/Lie-heterozygoten Rötelmäusen (Rötel- oder Waldwühlmaus, Clethrionomys glareolus) fanden Cartoni et al. nach Infektionen mit dem Maus-adaptierten Scrapie-Stamm 139A in den PrPsc-Aggregaten ähnliche Mengen beider PrP-Varianten.
MH Animals; Arvicolinae; Blotting, Western; Brain/*metabolism/pathology; Chromatography, High Pressure Liquid/*methods; Mass Spectrometry/*methods; Mice; PrPc Proteins/analysis/chemistry; PrPsc Proteins/analysis/chemistry; Prions/analysis/*chemistry
AD Istituto Superiore di Sanita, Department of Food Safety and Veterinary Public Health, Viale Regina Elena 299, 00161 Rome, Italy. cartoni@iss.it
SP englisch
PO Niederlande